The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:62500.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceC2orf28 is thought to be involved in apoptosis and may also be involved in hematopoietic development and differentiation. It is weakly expressed in hematopoietic cell lines. There are two named isoforms.
Cellular localizationCell membrane; Single pass membrane protein.