The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceBelongs to the GCF family and has two isoforms. Widely expressed in tissues and cell lines. Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters.
Anti-C2orf3 antibody (ab83407) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal lung lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 89 kDa Observed band size : 89 kDa
References for Anti-C2orf3 antibody (ab83407)
This product has been referenced in:
Yoshimoto R et al. Identification of a novel component C2ORF3 in the lariat-intron complex: lack of C2ORF3 interferes with pre-mRNA splicing via intron turnover pathway. Genes Cells19:78-87 (2014).
Read more (PubMed: 24304693) »