Detection of C2orf44 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118806 at 6 µg/mg lysate for IP. For WB detection an anti-C2orf44 antibody which recognizes a downstream epitope was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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