Overview

  • Product nameAnti-C3 / C3a antibody [2898]
    See all C3 / C3a primary antibodies
  • Description
    Mouse monoclonal [2898] to C3 / C3a
  • SpecificityThis antibody reacts with the C-terminus of C3a. It reacts with intact C3 as with C3a.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids

    CARASHLGLA

    of C3/ C3a (last nine amino acids of C3a-desArg).

  • Positive control
    • Human plasma

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notes0.2 µm filtered
  • ClonalityMonoclonal
  • Clone number2898
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution. Predicted molecular weight: 187.2 kDa.
    The starting working dilution is 1/10.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
      Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
      C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation.
      Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:8376604, PubMed:2909530, PubMed:9059512, PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750).
    • Tissue specificityPlasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods.
    • Involvement in diseaseComplement component 3 deficiency
      Macular degeneration, age-related, 9
      Hemolytic uremic syndrome atypical 5
      Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
    • Sequence similaritiesContains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications
      C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
      Phosphorylated by FAM20C in the extracellular medium.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acylation stimulating protein antibody
      • AHUS5 antibody
      • ARMD9 antibody
      • ASP antibody
      • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
      • C3 antibody
      • C3adesArg antibody
      • C3bc antibody
      • CO3_HUMAN antibody
      • Complement C3 alpha chain antibody
      • Complement C3 antibody
      • Complement C3c alpha'' chain fragment 2 antibody
      • Complement component 3 antibody
      • Complement component C3 antibody
      • Complement factor 3 antibody
      • CPAMD1 antibody
      • Plp antibody
      see all

    Anti-C3 / C3a antibody [2898] images

    • All lanes : Anti-C3 / C3a antibody [2898] (ab11874) at 1/200 dilution

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 187.2 kDa
      Observed band size : 187 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 115 kDa,71 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    References for Anti-C3 / C3a antibody [2898] (ab11874)

    This product has been referenced in:
    • Ecker SM  et al. Aqueous cytokine and growth factor levels do not reliably reflect those levels found in the vitreous. Mol Vis 17:2856-63 (2011). WB ; Human . Read more (PubMed: 22128233) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry and I'm sorry to hear that your customer is experiencing difficulty with this antibody. Without knowing more details about what your customer has already tried, I would suggest trying the primary at 1:500 and optimizing from ...

    Read More

    The antibodies our source has in their product range gave only one option for setting up an immunoassay to quantify complement C3a, but this option will also recognize C3. The option is: ab11874: monoclonal antibody to human C3/C3a (C-terminus, clo...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"