Overview

  • Product nameAnti-C3 / C3a antibody [474]
    See all C3 / C3a primary antibodies
  • Description
    Mouse monoclonal [474] to C3 / C3a
  • SpecificityThe antibody reacts with an epitope on C3a. It reacts with both intact C3, as with C3a. See references, multiple isoforms of this protein exist (multiple bands may appears in WB).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number474
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 187.2 kDa.

Target

  • FunctionC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation.
    Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:8376604, PubMed:2909530, PubMed:9059512, PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750).
  • Tissue specificityPlasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods.
  • Involvement in diseaseComplement component 3 deficiency
    Macular degeneration, age-related, 9
    Hemolytic uremic syndrome atypical 5
    Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
  • Sequence similaritiesContains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
    Phosphorylated by FAM20C in the extracellular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acylation stimulating protein antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • C3adesArg antibody
    • C3bc antibody
    • CO3_HUMAN antibody
    • Complement C3 alpha chain antibody
    • Complement C3 antibody
    • Complement C3c alpha'' chain fragment 2 antibody
    • Complement component 3 antibody
    • Complement component C3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • Plp antibody
    see all

Anti-C3 / C3a antibody [474] images

  • Lanes 1 & 4 : Anti-C3 / C3a antibody [474] (ab11872) at 1/250 dilution (in 2% BSA for 3 hours at 4°C)
    Lanes 2 & 5 : Anti-C3 / C3a antibody [474] (ab11872) at 1/500 dilution (in 2% BSA for 3 hours at 4°C)
    Lanes 3 & 6 : Anti-C3 / C3a antibody [474] (ab11872) at 1/1000 dilution (in 2% BSA for 3 hours at 4°C)

    Lane 1 : Human serum
    Lane 2 : Human serum
    Lane 3 : Human serum
    Lane 4 : Human serum
    Lane 5 : Human serum
    Lane 6 : Human serum

    Lysates/proteins at 20 µg per lane.

    Secondary
    An HRP-conjugated Sheep anti-mouse polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 187.2 kDa


    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Dr Koji Ueda

    See Abreview

References for Anti-C3 / C3a antibody [474] (ab11872)

This product has been referenced in:
  • Shi L  et al. Discovery and identification of potential biomarkers of pediatric Acute Lymphoblastic Leukemia. Proteome Sci 7:7 (2009). Read more (PubMed: 19291297) »
  • Li RX  et al. Localized-statistical quantification of human serum proteome associated with type 2 diabetes. PLoS ONE 3:e3224 (2008). WB ; Human . Read more (PubMed: 18795103) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Serum)
Loading amount 20 µg
Specification Serum
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

幸嗣 植田

Verified customer

Submitted Feb 16 2011

The antibodies our source has in their product range gave only one option for setting up an immunoassay to quantify complement C3a, but this option will also recognize C3. The option is: ab11874: monoclonal antibody to human C3/C3a (C-terminus, clo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"