• Product nameAnti-C3 / C3a antibody [474]
    See all C3 / C3a primary antibodies
  • Description
    Mouse monoclonal [474] to C3 / C3a
  • SpecificityThe antibody reacts with an epitope on C3a. It reacts with both intact C3, as with C3a. See references, multiple isoforms of this protein exist (multiple bands may appears in WB).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number474
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab11872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 187.2 kDa.


  • RelevanceComplement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptable to bacteria infection.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Acylation stimulating protein antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
    • C3 antibody
    • Complement C3 alpha chain antibody
    • Complement C3 antibody
    • Complement component 3 antibody
    • Complement component C3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • Plp antibody
    see all

Anti-C3 / C3a antibody [474] images

  • Lanes 1 & 4 : Anti-C3 / C3a antibody [474] (ab11872) at 1/250 dilution (in 2% BSA for 3 hours at 4°C)
    Lanes 2 & 5 : Anti-C3 / C3a antibody [474] (ab11872) at 1/500 dilution (in 2% BSA for 3 hours at 4°C)
    Lanes 3 & 6 : Anti-C3 / C3a antibody [474] (ab11872) at 1/1000 dilution (in 2% BSA for 3 hours at 4°C)

    Lane 1 : Human serum
    Lane 2 : Human serum
    Lane 3 : Human serum
    Lane 4 : Human serum
    Lane 5 : Human serum
    Lane 6 : Human serum

    Lysates/proteins at 20 µg per lane.

    An HRP-conjugated Sheep anti-mouse polyclonal at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 187.2 kDa

    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Dr Koji Ueda

    See Abreview

References for Anti-C3 / C3a antibody [474] (ab11872)

This product has been referenced in:
  • Shi L  et al. Discovery and identification of potential biomarkers of pediatric Acute Lymphoblastic Leukemia. Proteome Sci 7:7 (2009). Read more (PubMed: 19291297) »
  • Li RX  et al. Localized-statistical quantification of human serum proteome associated with type 2 diabetes. PLoS ONE 3:e3224 (2008). WB ; Human . Read more (PubMed: 18795103) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Serum)
Loading amount 20 µg
Specification Serum
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Koji Ueda

Verified customer

Submitted Feb 16 2011

The antibodies our source has in their product range gave only one option for setting up an immunoassay to quantify complement C3a, but this option will also recognize C3. The option is: ab11874: monoclonal antibody to human C3/C3a (C-terminus, clo...

Read More