The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a strong band of approximately 9 kDa(C3a), two weaker bands one at approximately 115 kDa (C3 alpha), the other at 36 - 50 kDa (unknown or partial cleavage C3).
1/2000 - 1/10000.
RelevanceComplement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptable to bacteria infection.