• Product nameAnti-C3 / C3a antibody
    See all C3 / C3a primary antibodies
  • Description
    Chicken polyclonal to C3 / C3a
  • SpecificityMay cross react with C3 / C3a from other mammalian species.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 678-692 of Mouse C3/ C3a

  • Positive control
    • Mouse plasma IHC-P: Mouse liver FFPE tissue sections.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein L purified
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas


Our Abpromise guarantee covers the use of ab48581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 187 kDa. Detects a strong band of approximately 9 kDa and two weaker bands at 115 and 25 - 36 kDa. Detection of the 9 kD band is improved by using PVDF membrane with 0.2um pores instead of 0.45um, and a Tricine gel buffer instead of Tris/glycine, according to customer feedback.
ELISA 1/2000 - 1/10000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • RelevanceComplement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptable to bacteria infection.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Acylation stimulating protein antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
    • C3 antibody
    • Complement C3 alpha chain antibody
    • Complement C3 antibody
    • Complement component 3 antibody
    • Complement component C3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • Plp antibody
    see all

Anti-C3 / C3a antibody images

  • IHC image of ab48581 staining in mouse liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48581, 10µg/ml, for 15 mins at room temperature. A goat anti-chicken biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-C3 / C3a antibody (ab48581)

This product has been referenced in:
  • Wang Y  et al. Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation 7:24 (2010). WB, ICC/IF ; Rat . Read more (PubMed: 20380727) »

See 1 Publication for this product

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