Recombinant
RabMAb

Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080)

Overview

  • Product name
    Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)]
    See all C3 / C3a primary antibodies
  • Description
    Rabbit monoclonal [EPR2987(2)(ABC)(B)] to C3 / C3a
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C3/ C3a aa 850-950. The exact sequence is proprietary.
    Database link: P01024

  • Positive control
    • Human plasma, fetal liver, fetal kidney, HepG2 and Human milk lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 50% Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR2987(2)(ABC)(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 187 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
      Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
      C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation.
      Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:8376604, PubMed:2909530, PubMed:9059512, PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750).
    • Tissue specificity
      Plasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods.
    • Involvement in disease
      Complement component 3 deficiency
      Macular degeneration, age-related, 9
      Hemolytic uremic syndrome atypical 5
      Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
    • Sequence similarities
      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications
      C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
      Phosphorylated by FAM20C in the extracellular medium.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acylation stimulating protein antibody
      • AHUS5 antibody
      • ARMD9 antibody
      • ASP antibody
      • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
      • C3 antibody
      • C3adesArg antibody
      • C3bc antibody
      • CO3_HUMAN antibody
      • Complement C3 alpha chain antibody
      • Complement C3 antibody
      • Complement C3c alpha'' chain fragment 2 antibody
      • Complement component 3 antibody
      • Complement component C3 antibody
      • Complement factor 3 antibody
      • CPAMD1 antibody
      • Plp antibody
      see all

    Images

    • All lanes : Anti-C3 / C3a antibody [EPR2987(2)(ABC)(B)] (ab171080) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human fetal liver tissue lysate
      Lane 3 : Human fetal kidney tissue lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Human milk lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 187 kDa
    • Western blot analysis on immunoprecipitation pellet from fetal kidney lysate (Lane 1) or 1X PBS (negative control) (Lane 2) labeling C3 / C3a using ab171080 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

       

      Lane 1 Primary: ab171080, 1/1000 dilution
      Sample: Fetal kidney tissue lysate
      Secondary: HRP-conjugated anti-rabbit IgG
      Lane 2 Primary: ab171080, 1/1000 dilution
      Sample: 1 X PBS
      Secondary: HRP-conjugated anti-rabbit IgG

       

    References

    ab171080 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171080.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up