Anti-C3 / C3b antibody [755] (ab11871)

Overview

• Product name
  Anti-C3 / C3b antibody [755]
  See all C3 / C3b primary antibodies
• Description
  Mouse monoclonal [755] to C3 / C3b
• Specificity
  This antibody reacts with C3 and C3b. See References multiple isoforms detected by WB.
• Tested applications
  Suitable for: IHC-P, WB, ICC/IFmore details
• Species reactivity
  Reacts with: Human, Cynomolgus monkey
  
• Immunogen

  C3 alpha chain, amino acids 1303-1663.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 0.1% BSA, PBS
• Concentration information loading...
• Purity
  Protein G purified
• Clonality
  Monoclonal
• Clone number
  755
• Isotype
  IgG2b
• Research areas
  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

  • Immunology
  • Innate Immunity
  • Complement
  • Classical Pathway

  • Immunology
  • Innate Immunity
  • Complement
  • Alternative Pathway

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • GPCR

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
• Immunohistochemistry kits
  • EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit (ab80436)

Applications

Target

• Function
  C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
  Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
• Tissue specificity
  Plasma.
• Involvement in disease
  Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
  Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
• Sequence similarities
  Contains 1 anaphylatoxin-like domain.
  Contains 1 NTR domain.
• Post-translational
  modifications
  C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
  Phosphorylation sites are present in the extracelllular medium.
• Cellular localization
  Secreted.

• Target information above from: UniProt accession P01024 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 718 Human
  • Omim: 120700 Human
  • SwissProt: P01024 Human
  • Unigene: 529053 Human
• Alternative names
  • Acylation-stimulating protein cleavage product antibody
  • AHUS5 antibody
  • ARMD9 antibody

  • ASP antibody
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
  • C3 antibody
  • CO3_HUMAN antibody
  • Complement C3 antibody
  • Complement C3 beta chain antibody
  • Complement C3c alpha' chain fragment 2 antibody
  • Complement component 3 antibody
  • Complement component C3 antibody
  • Complement factor 3 antibody
  • CPAMD1 antibody
  • Plp antibody

  see all

Protocols

• Western blot protocols
• Immunohistochemistry protocols
• Immunocytochemistry & immunofluorescence protocols