Overview

  • Product nameAnti-C3 / C3b antibody [755]
    See all C3 / C3b primary antibodies
  • Description
    Mouse monoclonal [755] to C3 / C3b
  • SpecificityThis antibody reacts with C3 and C3b. See References multiple isoforms detected by WB.
  • Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus Monkey
  • Immunogen

    C3 alpha chain, amino acids 1303-1663.

Properties

Applications

Our Abpromise guarantee covers the use of ab11871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 187.2 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 25254972

Target

  • FunctionC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similaritiesContains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acylation-stimulating protein cleavage product antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • CO3_HUMAN antibody
    • Complement C3 antibody
    • Complement C3 beta chain antibody
    • Complement C3c alpha' chain fragment 2 antibody
    • Complement component 3 antibody
    • Complement component C3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • Plp antibody
    see all

Anti-C3 / C3b antibody [755] images

  • ab11871 staining C3 / C3b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 32 minutes at 37°C. An undiluted biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of Cynomolgus Monkey spleen tissue, staining C3 / C3b with ab11871.

    Tissue was fixed with formaldehyde and blocked with blocking solution for 1 hour at 37°C; antigen retrieval was by heat mediation in a cell conditioner. Samples were incubated with primary antibody (1/50 in diluent) for 1 hour at 37°C. A biotinylated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

References for Anti-C3 / C3b antibody [755] (ab11871)

This product has been referenced in:
  • Murayama MA  et al. CTRP6 is an endogenous complement regulator that can effectively treat induced arthritis. Nat Commun 6:8483 (2015). Read more (PubMed: 26404464) »
  • Lee MS  et al. Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection. PLoS Pathog 10:e1004412 (2014). ICC/IF ; Human . Read more (PubMed: 25254972) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0 Bond Epitope Retrieval Solution 2 (ER2, Leica)
Permeabilization No
Specification Spleen
Blocking step 2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative 10% Neutral Buffered Formalin
Username

Abcam user community

Verified customer

Submitted Jun 15 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: CC1
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 26 2015

Thank you for contacting us.

There are following antibodies that are best match as per your request. Please check the datasheets.

ab11871, ab106108

I hope this information is helpful to you. Please do not hesitate to contact ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Cell Conditioner 1
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Dec 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tonsil and intestine)
Specification tonsil and intestine
Antigen retrieval step Other
Blocking step Background Buster as blocking agent for 16 minute(s) · Concentration: 0%
Username

Ms. Emilia Blake

Verified customer

Submitted Nov 21 2011

Thank you for your enquiry. I can confirm the ab11871 C3 / C3b antibody [755] will detect the C3 alpha and C3 beta chains. This is described in the publication which I have attached. I hope this will be helpful to you. If you have any f...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Apoptotic T cell leukemia line (Jurkat) coated wit)
Specification Apoptotic T cell leukemia line (Jurkat) coated wit
Preparation Cell harvesting/tissue preparation method: harvested from cell suspension, spun down,rinsed,
Sample buffer: PBS
Permeabilization No
Gating Strategy Gated on apoptotic Jurkat cells (High SSC/LowFSC)
Username

James Fraser

Verified customer

Submitted Jul 15 2011

Thank you for your message. I am pleased you are keen to submit an abreview and we look forward to recieving it. My sincere apologies, as I seem to have given you incorrect information earlier. I was looking at several antibodies for you and must h...

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Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rat has n...

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Thank you for your email. Some suggestions are ab9403 (Mouse IgG2b monoclonal (ME.04) - isotype control) or ab18469 (Mouse IgG2b, kappa monoclonal (MPC-11) - Isotype control). To view all of our isotype controls, just select "Tags and Markers" from...

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