Anti-C3 / C3b antibody [755] (ab11871)
Key features and details
- Mouse monoclonal [755] to C3 / C3b
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, Cynomolgus monkey
- Isotype: IgG2b
Overview
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Product name
Anti-C3 / C3b antibody [755]
See all C3 / C3b primary antibodies -
Description
Mouse monoclonal [755] to C3 / C3b -
Host species
Mouse -
Specificity
This antibody reacts with C3 and C3b. See References multiple isoforms detected by WB. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human, Cynomolgus monkey -
Immunogen
Full length native protein (purified) corresponding to Human C3/ C3b.
Database link: P01024 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
755 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 25254972
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IHC-P | (4) |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 25254972 |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
Sequence similarities
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
Post-translational
modificationsC3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 718 Human
- Omim: 120700 Human
- SwissProt: P01024 Human
- Unigene: 529053 Human
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Alternative names
- Acylation-stimulating protein cleavage product antibody
- AHUS5 antibody
- ARMD9 antibody
see all
Images
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Immunohistochemistry analysis of Paraffin embedded section of human tonsil tissue sections labeling C3 / C3b with ab11871 at 1/200.
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ab11871 staining C3 / C3b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 32 minutes at 37°C. An undiluted biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
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Immunohistochemical analysis of Cynomolgus Monkey spleen tissue, staining C3 / C3b with ab11871.
Tissue was fixed with formaldehyde and blocked with blocking solution for 1 hour at 37°C; antigen retrieval was by heat mediation in a cell conditioner. Samples were incubated with primary antibody (1/50 in diluent) for 1 hour at 37°C. A biotinylated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab11871 has been referenced in 23 publications.
- Rumsey JW et al. Classical Complement Pathway Inhibition in a "Human-On-A-Chip" Model of Autoimmune Demyelinating Neuropathies. Adv Ther (Weinh) 5:N/A (2022). PubMed: 36211621
- Riba M et al. Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages. Cell Biosci 12:177 (2022). PubMed: 36307854
- Jia L et al. Single-cell profiling of infiltrating B cells and tertiary lymphoid structures in the TME of gastric adenocarcinomas. Oncoimmunology 10:1969767 (2021). PubMed: 34513317
- Smith VM et al. A Functional Human-on-a-Chip Autoimmune Disease Model of Myasthenia Gravis for Development of Therapeutics. Front Cell Dev Biol 9:745897 (2021). PubMed: 34881241
- Fan S et al. Extensive Sub-RPE Complement Deposition in a Nonhuman Primate Model of Early-Stage Diabetic Retinopathy. Invest Ophthalmol Vis Sci 62:30 (2021). PubMed: 33749721