Anti-C3a Receptor antibody (ab7209)

Overview

  • Product nameAnti-C3a Receptor antibody
    See all C3a Receptor primary antibodies
  • Description
    Rabbit polyclonal to C3a Receptor
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 161-332 of Human C3a Receptor, corresponding to its second extracellular loop.

  • General notes


    C3a is an anaphylatoxin generated through activation of the complement C3, and is a potent chemoattractant for phagocytes, and stimulates chemotaxis and other leukocyte functions. C3a binds to eosinophils, basophils, neutrophils, and differentiated U937 cells, that all express receptors for C3a on their cell surface. The C3a receptor has been cloned and shown to be a G protein-coupled receptor with seven putative transmembrane domains (Crass 1996, Ames 1996). This receptor is characterized for its large extracellular loop between the fourth and fifth transmembrane domains (Roglic 1996). The loop can be readily detected using the rabbit anti-C3a receptor antibody.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesC3a is an anaphylatoxin generated through activation of the complement C3, and is a potent chemoattractant for phagocytes, and stimulates chemotaxis and other leukocyte functions. C3a binds to eosinophils, basophils, neutrophils, and differentiated U937 cells, that all express receptors for C3a on their cell surface. The C3a receptor has been cloned and shown to be a G protein-coupled receptor with seven putative transmembrane domains (Crass 1996, Ames 1996). This receptor is characterized for its large extracellular loop between the fourth and fifth transmembrane domains (Roglic 1996). The loop can be readily detected using the rabbit anti-C3a receptor antibody.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionReceptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
  • Tissue specificityWidely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AZ 3B antibody
    • AZ3B antibody
    • C3a anaphylatoxin chemotactic receptor antibody
    • C3A R antibody
    • C3A R1 antibody
    • C3a-R antibody
    • C3AR antibody
    • C3AR_HUMAN antibody
    • C3AR1 antibody
    • C3R1 antibody
    • Complement component 3 receptor 1 antibody
    • Complement component 3a receptor 1 antibody
    • HNFAG09 antibody
    see all

References for Anti-C3a Receptor antibody (ab7209)

ab7209 has not yet been referenced specifically in any publications.

Product Wall

As far as we are aware, this antibody has only been tested in flow cytometry.

I am afriad that I have no expertise in this area. I note from the datasheet that C3a binds to eosinophils, basophils, neutrophils, and differentiated U937 cells, that all express receptors for C3a on their cell surface. May I also suggest that you re...

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This antibody has not been tested for blocking activity in functional assays. However there are two antibodies in the Abcam catalogue directed against receptors which are known to have neutralising activity: ab7200 Rabbit polyclonal to rat CX3CR1 (fr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"