The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: 1/10 - 1/20. Use 10µl of the sugested working dilution to label 1x106 cells in 100µl.
IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Tissue specificityWidely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Post-translational modificationsAmong the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.