Overview

  • Product name
    Anti-C3c antibody (FITC)
    See all C3c primary antibodies
  • Description
    Rabbit polyclonal to C3c (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody reacts with human C3c complement and with the C3c part of C3 and C3b.
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Goat, Guinea pig, Cow, Cat, Dog, Human, Pig, Kangaroo, Mink
  • Immunogen

    C3c complement isolated from complement activated human serum.

  • Positive control
    • human kidney
  • General notes

    Fluorescein isothiocyanate (FITC) isomer 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab4212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Form
    Cleaved into the following 10 chains: 1) Complement C3 beta chain 2) Complement C3 alpha chain 3) C3a anaphylatoxin 4) Complement C3b alpha' chain 5) Complement C3c alpha' chain fragment 1 6) Complement C3dg fragment 7) Complement C3g fragment 8) Complement C3d fragment 9) Complement C3f fragment 10) Complement C3c alpha' chain fragment 2
  • Alternative names
    • acylation-stimulating protein cleavage product antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • C3a anaphylatoxin antibody
    • C3a antibody
    • C3b antibody
    • CO3_HUMAN antibody
    • Complement C3 antibody
    • Complement C3c alpha' chain fragment 2 antibody
    • Complement C3c antibody
    • Complement component 3 antibody
    • Complement component C3 antibody
    • Complement component C3a antibody
    • Complement component C3b antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • Prepro C3 antibody
    see all

Images

  • ICC/IF image of ab4212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4212, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Bahia El Idrissi N  et al. Complement activation at the motor end-plates in amyotrophic lateral sclerosis. J Neuroinflammation 13:72 (2016). Read more (PubMed: 27056040) »
  • Zhang Y  et al. Effect of the glycosyltransferases on the capsular polysaccharide synthesis of Streptococcus suis serotype 2. Microbiol Res 185:45-54 (2016). Read more (PubMed: 26946377) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry and your interest.

I can confirm that the Ig concentration for this antibody is 1.4g/l.

If you need any further assistance in the future, please do not hesitate to contact me.

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Included in...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (ovary)
Specification
ovary
Preparation
Cell harvesting/tissue preparation method: trypsinisation
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
live cells
Username

Abcam user community

Verified customer

Submitted Dec 05 2007

I can confirm that the recommended positive control must be human kidney and that the tissue should be fixed in acetone. Unfixed tissue may not be suitable for staining. I hope this information helps, please do not hesitate to contact us if you n...

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I'm sorry to hear you are having a problem with ab4212. I would like to suggest the following modifications to your protocol: -make sure the antibody is protected from the light -fix the frozen tissue sections with ice cold acetone or methanol fo...

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