The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionServes as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.
Tissue specificityHighly expressed in testis and at lower levels in the submandibular salivary gland.
ab101996 at 1 µg/ml staining C3IP1 in HeLa cell lysate immunoprecipitated using ab101994 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds
References for Anti-C3IP1 antibody (ab101994)
has not yet been referenced specifically in any publications.
Publishing research using ab101994? Please let us know so that we can cite the reference in this datasheet.
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