Overview

  • Product name
    Anti-C3orf10 antibody
  • Description
    Rabbit polyclonal to C3orf10
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    A 14-22 amino acid region within synthetic peptide: SSIKKIADFL NSFDMSCRSR LATLNEKLTA LERRIEYIEA RVTKGETLT, corresponding to amino acids 27-49 of Human C3orf10

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab65445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 9 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Relevance
    C3orf10 is involved in regulation of actin and microtubule organization and is part of a WAVE complex that activates the Arp2/3 complex.
  • Cellular localization
    Cytoplasm, cytoskeleton
  • Database links
  • Alternative names
    • BRICK1, SCAR/WAVE actin nucleating complex subunit antibody
    • BRICK1, SCAR/WAVE actin nucleating complex subunit, homolog antibody
    • BRK1 antibody
    • Brk1-like antibody
    • C3orf10 chromosome 3 open reading frame 10 antibody
    • C3ORF10 GENE antibody
    • Chromosome 3 open reading frame 10 antibody
    • Haematopoietic stem/progenitor cell protein 300 antibody
    • hHBrk1 antibody
    • HSPC300 antibody
    • MDS027 antibody
    • Probable protein BRICK1 antibody
    • Protein BRICK1 antibody
    see all

Images

  • Anti-C3orf10 antibody (ab65445) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 9 kDa
  • ab65445 at 4µg/ml, staining C3orf10 in paraffin embedded hepatocytes tissue from human liver (indicated by arrows) by Immunohistochemistry.

References

ab65445 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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