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This antibody reacts with intact C4, C4b and C4d.
Our Abpromise guarantee covers the use of ab11863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10. Predicted molecular weight: 192 kDa.|
|Flow Cyt||1/10. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
Immunohistochemistry (Frozen sections) analysis of mouse kidney allografts (left) and normal kidney tissue labelling C4 with ab11863 at 1/50. Staining seen predominantly in peritubular capillary walls. Magnification: X250.
4μm frozen sections were fixed in acetone and blocked in normal goat serum. A FITC-conjugated goat anti-rat IgG was used as the secondary antibody.