Overview

  • Product nameAnti-C4 antibody [16D2]
    See all C4 primary antibodies
  • Description
    Rat monoclonal [16D2] to C4
  • SpecificityAntibody cross reacts with C4, C4b and C4d.
  • Tested applicationsSuitable for: WB, IHC-Fr, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    This antibody reacts with intact C4, C4b and C4d.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number16D2
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10. Predicted molecular weight: 192 kDa.
IHC-Fr 1/10.
IP 1/10.
Flow Cyt 1/10. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Acidic C4 antibody
    • Acidic complement C4 antibody
    • Basic C4 antibody
    • Basic complement C4 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4, Chido form antibody
    • C4, Rodgers from antibody
    • C4A anaphylatoxin antibody
    • C4A antibody
    • C4A2 antibody
    • C4A3 antibody
    • C4A4 antibody
    • C4A6 antibody
    • C4AD antibody
    • C4B antibody
    • C4B_2 antibody
    • C4B1 antibody
    • C4B12 antibody
    • C4B2 antibody
    • C4B3 antibody
    • C4BD antibody
    • C4F antibody
    • C4S antibody
    • CH antibody
    • Chido form of C4 antibody
    • CO4 antibody
    • CO4A_HUMAN antibody
    • Complement C4 gamma chain antibody
    • complement C4-A antibody
    • complement C4-B antibody
    • complement C4-B-like antibody
    • complement C4B1a antibody
    • Complement component 4A (Rodgers blood group) antibody
    • Complement component 4A antibody
    • complement component 4B (Chido blood group), copy 2 antibody
    • Complement component 4B (Childo blood group) antibody
    • Complement component 4B antibody
    • Complement component 4F antibody
    • Complement component 4S antibody
    • CPAMD2 antibody
    • CPAMD3 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all

Anti-C4 antibody [16D2] images

  • Immunohistochemistry (Frozen sections) analysis of mouse kidney allografts (left) and normal kidney tissue labelling C4 with ab11863 at 1/50. Staining seen predominantly in peritubular capillary walls. Magnification: X250.

    4μm frozen sections were fixed in acetone and blocked in normal goat serum. A FITC-conjugated goat anti-rat IgG was used as the secondary antibody.

References for Anti-C4 antibody [16D2] (ab11863)

This product has been referenced in:
  • Garland DL  et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol Genet N/A:N/A (2013). Mouse . Read more (PubMed: 23943789) »
  • Cohen D  et al. Classical complement activation as a footprint for murine and human antiphospholipid antibody-induced fetal loss. J Pathol 225:502-11 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21688269) »

See all 5 Publications for this product

Product Wall

Assuming you have not already tried this clone, 16D2, the antibody ab11863 should be capable of detecting mouse C4, but we do not know how much is expected to be present in brain tissue. We recommend a positive control of, for instance, mouse kidney. T...

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Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (kidney)
Specification kidney
Treatment allografted
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2014

Thank you for contacting us. I have been doing a little research into C4 and the antibodies that we currently have available against this target. As discussed on the phone, ab89537 recognises mouse C4d and C4 and has been tested in flow cytomet...

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Thank you for contacting us. The following reference has used the antibody and gives details of its characterization: Murata, K et al; Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"