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This antibody reacts with intact C4, C4b and C4d.
Our Abpromise guarantee covers the use of ab11863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10. Predicted molecular weight: 192 kDa.|
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Immunohistochemistry (Frozen sections) analysis of mouse kidney allografts (left) and normal kidney tissue labelling C4 with ab11863 at 1/50. Staining seen predominantly in peritubular capillary walls. Magnification: X250.
4μm frozen sections were fixed in acetone and blocked in normal goat serum. A FITC-conjugated goat anti-rat IgG was used as the secondary antibody.