The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab23478 reacts strongly with C4. A strong reaction is seen with normal human serum, and no reaction is seen with serum depleted of C4, when tested in sandwich ELISA in combination with a polyclonal antibody against C4. ab23478 also reacts with C4b deposited on coated antibody molecules.
Use at an assay dependent concentration. Predicted molecular weight: 193 kDa.
In Western blotting after SDS-PAGE, ab23478 reacts with C4, in reduced as well as non-reduced forms.