Overview

  • Product nameAnti-C4 antibody
    See all C4 primary antibodies
  • Description
    Goat polyclonal to C4
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human)

  • Positive control
    • FFPE human normal liver tissue sections This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 193 kDa.
ELISA Use a concentration of 1 - 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Acidic C4 antibody
    • Acidic complement C4 antibody
    • Basic C4 antibody
    • Basic complement C4 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4, Chido form antibody
    • C4, Rodgers from antibody
    • C4A anaphylatoxin antibody
    • C4A antibody
    • C4A2 antibody
    • C4A3 antibody
    • C4A4 antibody
    • C4A6 antibody
    • C4AD antibody
    • C4B antibody
    • C4B_2 antibody
    • C4B1 antibody
    • C4B12 antibody
    • C4B2 antibody
    • C4B3 antibody
    • C4BD antibody
    • C4F antibody
    • C4S antibody
    • CH antibody
    • Chido form of C4 antibody
    • CO4 antibody
    • CO4A_HUMAN antibody
    • Complement C4 gamma chain antibody
    • complement C4-A antibody
    • complement C4-B antibody
    • complement C4-B-like antibody
    • complement C4B1a antibody
    • Complement component 4A (Rodgers blood group) antibody
    • Complement component 4A antibody
    • complement component 4B (Chido blood group), copy 2 antibody
    • Complement component 4B (Childo blood group) antibody
    • Complement component 4B antibody
    • Complement component 4F antibody
    • Complement component 4S antibody
    • CPAMD2 antibody
    • CPAMD3 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all

Anti-C4 antibody images

  • IHC image of C4 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47788, 5µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab47788 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab47788 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-C4 antibody (ab47788)

This product has been referenced in:
  • Avirutnan P  et al. Antagonism of the complement component C4 by flavivirus nonstructural protein NS1. J Exp Med 207:793-806 (2010). IP . Read more (PubMed: 20308361) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"