• Product name
    Anti-C4 binding protein antibody
    See all C4 binding protein primary antibodies
  • Description
    Rabbit polyclonal to C4 binding protein
  • Specificity
    This antibody is specific to the alpha chain of the C4 Binding Protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 548-597 (QVLTGKRLMQ CLPNPEDVKM ALEVYKLSLE IEQLELQRDS ARQSTLDKEL) of human C4 binding protein alpha chain precursor

  • Positive control
    • MCF7 cell lysate.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab83755 is purified by a peptide affinity chromatography method.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab83755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.
  • Sequence similarities
    Contains 3 Sushi (CCP/SCR) domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • C4b binding protein beta chain antibody
    • C4b-binding protein beta chain antibody
    • C4BP antibody
    • C4BPB antibody
    • C4BPB_HUMAN antibody
    • Complement component 4 binding protein beta antibody
    • Complement component 4 binding protein beta chain antibody
    • Complement component 4-binding protein beta antibody
    • OTTHUMP00000034288 antibody
    • OTTHUMP00000034289 antibody
    • OTTHUMP00000034375 antibody
    • OTTHUMP0000003474 antibody
    • Proline rich protein antibody
    • PRP antibody
    see all

Anti-C4 binding protein antibody images

  • Anti-C4 binding protein antibody (ab83755) at 1 µg/ml + MCF7 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 67 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <22 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab83755 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83755, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-C4 binding protein antibody (ab83755)

ab83755 has not yet been referenced specifically in any publications.

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