Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 193 kDa).

Complement C4-A circulates in blood as a disulphide linked trimer of an alpha, beta and gamma chain (95, 75 and 33 kDa respectively). Our immunogen sequence is within the region corresponding to the alpha chain (95 kDa).

Target

  • FunctionC4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Involvement in diseaseDefects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
  • Sequence similaritiesContains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acidic complement C4 antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C4a anaphylatoxin antibody
    • C4A antibody
    • C4AD antibody
    • CO4A_HUMAN antibody
    • Complement C4 A antibody
    • Complement C4 gamma chain antibody
    • Complement component 4A antibody
    • Complement component 4A preproprotein antibody
    • CPAMD2 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all

Anti-C4a antibody images

  • Anti-C4a antibody (ab87429) at 2 µg/ml + Human Plasma Total Protein Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 193 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Complement C4-A circulates in blood as a disulphide linked trimer of an alpha, beta and gamma chain (95, 75 and 33 kDa respectively). Our immunogen sequence is within the region corresponding to the alpha chain (95 kDa).

References for Anti-C4a antibody (ab87429)

This product has been referenced in:
  • Hyung SW  et al. A serum protein profile predictive of the resistance to neoadjuvant chemotherapy in advanced breast cancers. Mol Cell Proteomics 10:M111.011023 (2011). WB ; Human . Read more (PubMed: 21799047) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"