Overview

  • Product name
    Anti-C4A antibody [C4D204]
    See all C4A primary antibodies
  • Description
    Mouse monoclonal [C4D204] to C4A
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human C4A. Recombinant Human Complement 4d protein.
    Database link: P0C0L4

  • Positive control
    • kidney Transplant Tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Bioreactor concentrate
  • Clonality
    Monoclonal
  • Clone number
    C4D204
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/100 - 1/200.
ICC/IF 1/50 - 1/100.

Target

  • Relevance
    C4A is the acidic form of complement factor 4, part of the classical activation pathway. Human complement component C4 is polymorphic at two loci, C4A and C4B. The C4A alleles carry the Rodgers (Rg) while the C4B alleles carry the Chido (Ch) blood group antigens. C4 (either allele A or B) protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Acidic C4 antibody
    • Acidic complement C4 antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 2 antibody
    • C4 antibody
    • C4A anaphylatoxin antibody
    • C4A antibody
    • C4A2 antibody
    • C4A3 antibody
    • C4A4 antibody
    • C4A6 antibody
    • C4AD antibody
    • C4S antibody
    • CO4 antibody
    • Complement C4-A antibody
    • Complement component 4A antibody
    • Complement component 4A (Rodgers blood group) antibody
    • CPAMD2 antibody
    • RG antibody
    • Rodgers blood group antibody
    • Rodgers form of C4 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded kidney transplant tissue  labeling C4A with ab187278 at 1/100 dilution for 30 minutes at RT.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded kidney transplant tissue labeling C4A with ab187278 at 1/100 dilution for 30 minutes at RT.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded kidney transplant tissue labeling C4A with ab187278 at 1/100 dilution for 30 minutes at RT.

References

ab187278 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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