Recombinant
RabMAb

Anti-C4a antibody [EPR11241] (ab170917)

Overview

  • Product name
    Anti-C4a antibody [EPR11241]
    See all C4a primary antibodies
  • Description
    Rabbit monoclonal [EPR11241] to C4a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human C4a. The exact sequence is proprietary.
    Database link: P0C0L4

  • Positive control
    • Human angioneoplasm lysate, HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11241
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 193 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC or IP.
  • Target

    • Function
      C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
      Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Involvement in disease
      Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
    • Sequence similarities
      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications
      Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
      N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acidic complement C4 antibody
      • C3 and PZP like alpha 2 macroglobulin domain containing protein 2 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
      • C4a anaphylatoxin antibody
      • C4A antibody
      • C4AD antibody
      • CO4A_HUMAN antibody
      • Complement C4 A antibody
      • Complement C4 gamma chain antibody
      • Complement component 4A antibody
      • Complement component 4A preproprotein antibody
      • CPAMD2 antibody
      • RG antibody
      • Rodgers form of C4 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized HepG2 cells labeling C4a with ab170917 at 1/100 (red)  or a rabbit IgG negative (green).

    • All lanes : Anti-C4a antibody [EPR11241] (ab170917) at 1/1000 dilution

      Lane 1 : Human angioneoplasm lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat ant-rabbit HRP conjugated antibody at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 193 kDa

    References

    ab170917 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170917.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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