• Product nameAnti-C4b antibody
    See all C4b primary antibodies
  • Description
    Chicken polyclonal to C4b
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Human C4b protein.

  • Positive control
    • Human Placenta tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • ClonalityPolyclonal
  • IsotypeIgY
  • Research areas


Our Abpromise guarantee covers the use of ab118887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 193 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.1µg for 106 cells. ab37382-Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.


  • FunctionC4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Sequence similaritiesContains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic complement C4 antibody
    • C2B5 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4b antibody
    • C4B1 antibody
    • C4B2 antibody
    • C4B3 antibody
    • CO4B_HUMAN antibody
    • Complement C4 gamma chain antibody
    • Complement component 4B antibody
    • CPAMD3 antibody
    see all

Anti-C4b antibody images

  • ab118887, at 20 µg/ml, staining C4b in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-C4b antibody (ab118887)

ab118887 has not yet been referenced specifically in any publications.

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