• Product nameAnti-C4b antibody
    See all C4b primary antibodies
  • Description
    Mouse monoclonal to C4b
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human, Chimpanzee
  • Immunogen

    Recombinant fragment: NNRQIRGLEE ELQFSLGSKI NVKVGGNSKG TLKVLRTYNV LDMKNTTCQD LQIEVTVKGH VEYTMEANED YEDYEYDELP AKDDPDAPLQ PVTPLQLFEG, corresponding to amino acids 1347-1447 of Human C4b (NP_000583) with tag.

  • Positive control
    • Recombinant tagged human C4b fragment.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab76504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 193 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionC4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
      Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Sequence similaritiesContains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic complement C4 antibody
      • C2B5 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
      • C4b antibody
      • C4B1 antibody
      • C4B2 antibody
      • C4B3 antibody
      • CO4B_HUMAN antibody
      • Complement C4 gamma chain antibody
      • Complement component 4B antibody
      • CPAMD3 antibody
      see all

    Anti-C4b antibody images

    • Anti-C4b antibody (ab76504) at 1 µg/ml + Recombinant tagged human C4b fragment at 0.2 µg

      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 193 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    References for Anti-C4b antibody (ab76504)

    ab76504 has not yet been referenced specifically in any publications.

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