Overview

  • Product name
    Anti-C4b antibody [EPR11203]
    See all C4b primary antibodies
  • Description
    Rabbit monoclonal [EPR11203] to C4b
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human C4b (UniProt P0C0L5).

  • Positive control
    • Human serum, Human fetal liver and HepG2 lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11203
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 193 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
      Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Sequence similarities
      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications
      Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic complement C4 antibody
      • C2B5 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
      • C4b antibody
      • C4B1 antibody
      • C4B2 antibody
      • C4B3 antibody
      • CO4B_HUMAN antibody
      • Complement C4 gamma chain antibody
      • Complement component 4B antibody
      • CPAMD3 antibody
      see all

    Images

    • All lanes : Anti-C4b antibody [EPR11203] (ab168358) at 1/1000 dilution

      Lane 1 : Human serum lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 193 kDa

    • Flow cytometric analysis of permeabilized HepG2 cells labeling C4b with ab168358 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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