Overview

  • Product name
  • Description
    Rabbit polyclonal to C4BPA
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 454-592 of Human C4BPA (P04003).

  • Positive control
    • Human small intestine tissue; Human Liver and Human Tonsil lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

  • Function
    Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.
  • Tissue specificity
    Chylomicrons in the plasma.
  • Sequence similarities
    Contains 8 Sushi (CCP/SCR) domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C4b-binding protein alpha chain antibody
    • C4bp antibody
    • C4bpa antibody
    • C4BPA_HUMAN antibody
    • Complement component 4 binding protein alpha antibody
    • Complement component 4 binding protein antibody
    • Proline-rich protein antibody
    • PrP antibody
    • RP11-164O23.4 antibody
    see all

Images

  • All lanes : Anti-C4BPA antibody (ab121123) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121123 at 1/475 dilution staining C4BPA in Paraffin-emebedded Human small intestine tissue by Immunohistochemistry. Note: distinct positivity in plasma.

References

ab121123 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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