Overview

  • Product name
    Anti-C4c+C4b antibody (FITC)
  • Description
    Rabbit polyclonal to C4c+C4b (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody reacts with human C4, C4b and C4c but does not react with the C4d epitope.
  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    C4c complement isolated from complement activated human serum.

  • General notes

    Fluorescein isothiocyanate (FITC) isomer 1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/100.

Incubate for 30 - 60 min at room temperature. Optimal dilutions / concentrations should be determined by the end user.

IHC-P 1/50 - 1/100.

Incubate for 30 - 60 min at room temperature. Optimal dilutions / concentrations should be determined by the end user.

Target

  • Relevance
    C4 plays a central role in the activation of the classical pathway of the complement system. Human complement component C4 is polymorphic at two loci, C4A and C4B. The C4A alleles carry the Rodgers (Rg) while the C4B alleles carry the Chido (Ch) blood group antigens. C4 (either allele A or B) protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Acidic complement C4 antibody
    • Basic complement C4 antibody
    • CH antibody
    • Chido blood group antibody
    • Complement component 4A antibody
    • Complement component 4B antibody
    • RG antibody
    • Rodgers blood group antibody
    see all

References for Anti-C4c+C4b antibody (FITC) (ab4216)

ab4216 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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