• Product nameAnti-C4d antibody
    See all C4d primary antibodies
  • Description
    Rabbit polyclonal to C4d
  • SpecificityThis antibody is specific for a sequence in the C4d domain of native C4 and C4d. Although not tested for the C4d domain of C4b, it very likely binds this as well.
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 1223/1237 of Human C4

  • Positive control
    • Glomerular mesangial tissue


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab36075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/80. Antigen retrieval with a citrate buffer system (or equivalent) is recommended.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/20 - 1/80.
IHC-Fr 1/20 - 1/80.


  • FunctionC4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Involvement in diseaseDefects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
  • Sequence similaritiesContains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • acidic C4 antibody
    • Acidic complement C4 antibody
    • basic C4 antibody
    • basic complement C4 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4 antibody
    • C4, chido form antibody
    • C4, Rodgers form antibody
    • C4-1 antibody
    • C4a anaphylatoxin antibody
    • C4A antibody
    • C4A2 antibody
    • C4A3 antibody
    • C4A4 antibody
    • C4A6 antibody
    • C4AD antibody
    • C4b antibody
    • C4B_2 antibody
    • C4B1 antibody
    • C4B12 antibody
    • C4B2 antibody
    • C4B3 antibody
    • C4B5 antibody
    • C4BD antibody
    • C4d antibody
    • C4d fragment antibody
    • C4F antibody
    • C4S antibody
    • CH antibody
    • Chido form of C4 antibody
    • CO4 antibody
    • CO4A_HUMAN antibody
    • Complement C4 gamma chain antibody
    • complement C4-A antibody
    • complement C4-B antibody
    • complement C4-B-like antibody
    • complement C4B1a antibody
    • Complement component 4A antibody
    • complement component 4A (Rodgers blood group) antibody
    • Complement component 4B antibody
    • complement component 4B (Chido blood group) antibody
    • complement component 4B (Chido blood group), copy 2 antibody
    • complement component 4B (Chido blood group)-like antibody
    • Complement component 4F antibody
    • Complement component 4S antibody
    • CPAMD2 antibody
    • CPAMD3 antibody
    • DADB-112B14.11 antibody
    • MGC164979 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all

Anti-C4d antibody images

  • Immunohostochemical analysis of C4d expression in paraffin embedded formalin fixed human glomerular mesangial tissue, using 1/40 ab36075. Staining developed using high sensitivity Avidin/Biotin detection system.
  • ab36075 staining C4d in human kidney cells by Immunocytochemistry/ Immunofluorescence (snap frozen). The cells were acetone fixed. Samples were incubated with primary antibody at 1/60 for 1 hour at 25°C. The secondary antibody used was an IgG conjugated to Alexa Fluor® 488.

References for Anti-C4d antibody (ab36075)

This product has been referenced in:
  • Almarastani M  et al. Salvaging of severely ruptured living-related renal allograft secondary to acute antibody mediated rejection. Int J Surg Case Rep 5:723-6 (2014). IHC-P ; Human . Read more (PubMed: 25216193) »
  • Sansonno D  et al. Role of the receptor for the globular domain of c1q protein in the pathogenesis of hepatitis C virus-related cryoglobulin vascular damage. J Immunol 183:6013-20 (2009). IHC-P ; Human . Read more (PubMed: 19828637) »

See all 2 Publications for this product

Product Wall

Thank you for the reply.

Could you please confirm whether either of the vials of ab36075 purchased showed any staining (one of the 20xxxx and the one from the xxxxx)?

Also, I asked for some feedback of how the antibodies had been stor...

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Thank you for contacting us. The antibody is supplied in liquid form and does not have to be reconstituted. The vial should contain 50 ul (0.2 mg/ml), if you do not see it you should spin it down. I can direct you to a starting dilution if you can tell...

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Thank you for your reply.

Having reviewed this case it seems that using the protocol the customer has applied the antibody should be producing staining.

May I ask, how has the antibody been stored? At what temperature and was it ali...

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Thank you very much for having followed up with the customer and provided this additional information.

When looking through the protocol, I was surprised about the information on the secondary antibody. The customer mentioned to have used a r...

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We have previously been in contact regarding ab36075, which was not providing satisfactory results.

A free of charge replacement vial was issued and I am writing to inquire if the replacement product has worked. I would appreciate if you could...

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Your credit note ID is XXXXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used...

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Thank you for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I ap...

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Thank you for this additional information.

I would be pleased to investigate this issue further to see whether we could provide some protocol tips. Can you please therefore send us the protocol details with help of the filled questionnaire (i...

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Thank you for your response.

I am sorry to hear that you have been experiencing problems when using our products. Our anti-Twist antibody in guaranteed to work in mouse tissues in IHC-Fr. We have added recommended dilutions or specific proto...

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Thank you for contacting us.

I am sorry to hearthat a customer is experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
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