Overview

  • Product nameAnti-C4d antibody
    See all C4d primary antibodies
  • Description
    Rabbit polyclonal to C4d
  • SpecificityThis antibody is unique in that it has been shown to detect both the C4 protein at 200kDa as well as the 40kDa C4d protein.
  • Tested applicationsSuitable for: ICC, Flow Cyt, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    KLH conjugated syn-thetic mouse C4 peptide fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Flow Cyt
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 200,41 kDa (predicted molecular weight: 193 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionC4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
      Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Involvement in diseaseDefects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
    • Sequence similaritiesContains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications
      Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
      N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • acidic C4 antibody
      • Acidic complement C4 antibody
      • basic C4 antibody
      • basic complement C4 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
      • C4 antibody
      • C4, chido form antibody
      • C4, Rodgers form antibody
      • C4-1 antibody
      • C4a anaphylatoxin antibody
      • C4A antibody
      • C4A2 antibody
      • C4A3 antibody
      • C4A4 antibody
      • C4A6 antibody
      • C4AD antibody
      • C4b antibody
      • C4B_2 antibody
      • C4B1 antibody
      • C4B12 antibody
      • C4B2 antibody
      • C4B3 antibody
      • C4B5 antibody
      • C4BD antibody
      • C4d antibody
      • C4d fragment antibody
      • C4F antibody
      • C4S antibody
      • CH antibody
      • Chido form of C4 antibody
      • CO4 antibody
      • CO4A_HUMAN antibody
      • Complement C4 gamma chain antibody
      • complement C4-A antibody
      • complement C4-B antibody
      • complement C4-B-like antibody
      • complement C4B1a antibody
      • Complement component 4A antibody
      • complement component 4A (Rodgers blood group) antibody
      • Complement component 4B antibody
      • complement component 4B (Chido blood group) antibody
      • complement component 4B (Chido blood group), copy 2 antibody
      • complement component 4B (Chido blood group)-like antibody
      • Complement component 4F antibody
      • Complement component 4S antibody
      • CPAMD2 antibody
      • CPAMD3 antibody
      • DADB-112B14.11 antibody
      • MGC164979 antibody
      • RG antibody
      • Rodgers form of C4 antibody
      see all

    Anti-C4d antibody images



    • Predicted band size : 193 kDa
      Observed band size : 41,200 kDa (why is the actual band size different from the predicted?)

      Western blot analysis using ab89537. Serum from a C57BL6 mouse was aged for 0, 1 and 4 days at 37degrees C to allow cleavage of C4 to C4d. Blots reacted with ab89537 yielded both a 200 kDa band as well as a 41 kDa band corresponding to the molecular weight of C4d.

    References for Anti-C4d antibody (ab89537)

    ab89537 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. I have been doing a little research into C4 and the antibodies that we currently have available against this target. As discussed on the phone, ab89537 recognises mouse C4d and C4 and has been tested in flow cytomet...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"