Recombinant
RabMAb

Anti-C4d antibody [SP91] - C-terminal (ab183311)

Overview

  • Product name
    Anti-C4d antibody [SP91] - C-terminal
    See all C4d primary antibodies
  • Description
    Rabbit monoclonal [SP91] to C4d - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human C4d (C terminal). The exact sequence is proprietary.
    Database link: P0C0L5

  • Positive control
    • Human tonsil tissue
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...
  • Purification notes
    Purified from TCS by protein A/G.
  • Clonality
    Monoclonal
  • Clone number
    SP91
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab183311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform Antigen Retrieval by boiling tissue sections in 1mM EDTA, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Function
    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Involvement in disease
    Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
  • Sequence similarities
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • acidic C4 antibody
    • Acidic complement C4 antibody
    • basic C4 antibody
    • basic complement C4 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4 antibody
    • C4, chido form antibody
    • C4, Rodgers form antibody
    • C4-1 antibody
    • C4a anaphylatoxin antibody
    • C4A antibody
    • C4A2 antibody
    • C4A3 antibody
    • C4A4 antibody
    • C4A6 antibody
    • C4AD antibody
    • C4b antibody
    • C4B_2 antibody
    • C4B1 antibody
    • C4B12 antibody
    • C4B2 antibody
    • C4B3 antibody
    • C4B5 antibody
    • C4BD antibody
    • C4d antibody
    • C4d fragment antibody
    • C4F antibody
    • C4S antibody
    • CH antibody
    • Chido form of C4 antibody
    • CO4 antibody
    • CO4A_HUMAN antibody
    • Complement C4 gamma chain antibody
    • complement C4-A antibody
    • complement C4-B antibody
    • complement C4-B-like antibody
    • complement C4B1a antibody
    • Complement component 4A antibody
    • complement component 4A (Rodgers blood group) antibody
    • Complement component 4B antibody
    • complement component 4B (Chido blood group) antibody
    • complement component 4B (Chido blood group), copy 2 antibody
    • complement component 4B (Chido blood group)-like antibody
    • Complement component 4F antibody
    • Complement component 4S antibody
    • CPAMD2 antibody
    • CPAMD3 antibody
    • DADB-112B14.11 antibody
    • MGC164979 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all

Images

  • Immunohistochemical analysis of Human tonsil tissue labeling C4d using ab183311 at a 1/100 dilution

  • Immunohistochemical analysis of Human tonsil tissue labeling C4d using ab183311 at a 1/100 dilution.

References

ab183311 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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