Synthetic peptide corresponding to Human C5/ C5a aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin (KLH).
(Peptide available as
This antibody binds specifically to the alpha / alpha' chain of C5, which forms part of C5b
Our Abpromise guarantee covers the use of ab46168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 188 kDa).Can be blocked with C5 / C5b peptide (ab46167). |
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: C5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: A549 whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab46168 observed at 170 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab46168 was shown to recognize C5 when C5 knockout samples were used, along with additional cross-reactive bands. Wild-type and C5 knockout samples were subjected to SDS-PAGE. Ab46168 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10003 dilution for 1 hour at room temperature before imaging.
Complement component C5 protein is comprised of an alpha and beta chain which have predicted molecular weights of 115 kDa and 75 kDa respectively. ab46168 specifically detects the C5 alpha chain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"