Overview

  • Product name
    Anti-C5 / C5a antibody
    See all C5 / C5a primary antibodies
  • Description
    Rabbit polyclonal to C5 / C5a
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human C5/ C5a aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab46167)

  • Positive control
    • This antibody gave a positive signal in purified human Complement C5 protein.
  • General notes


    This antibody binds specifically to the alpha / alpha' chain of C5, which forms part of C5b

Properties

Applications

Our Abpromise guarantee covers the use of ab46168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 188 kDa).Can be blocked with C5 / C5b peptide (ab46167).

Target

  • Function
    Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • Involvement in disease
    Complement component 5 deficiency
    An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • Sequence similarities
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Anaphylatoxin C5a analog antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
      • C5 antibody
      • C5a antibody
      • CO5_HUMAN antibody
      • Complement C5 alpha'' chain antibody
      • Complement C5 antibody
      • Complement component C5 antibody
      • CPAMD4 antibody
      see all

    Images

    • Western blot - Anti-C5 / C5a antibody (ab46168)
      Western blot - Anti-C5 / C5a antibody (ab46168)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: C5 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: A549 whole cell lysate (20 µg)
      Lane 4: HepG2 whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab46168 observed at 170 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab46168 was shown to recognize C5 when C5 knockout samples were used, along with additional cross-reactive bands. Wild-type and C5 knockout samples were subjected to SDS-PAGE. Ab46168 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10003 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-C5 / C5a antibody (ab46168)
      Western blot - Anti-C5 / C5a antibody (ab46168)
      Anti-C5 / C5a antibody (ab46168) at 1 µg/ml + Human Complement C5 Protein at 0.1 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 188 kDa
      Observed band size: 115 kDa (why is the actual band size different from the predicted?)



      Complement component C5 protein is comprised of an alpha and beta chain which have predicted molecular weights of 115 kDa and 75 kDa respectively.  ab46168 specifically detects the C5 alpha chain.

    References

    This product has been referenced in:
    • Bettoni S  et al. Insights into the Effects of Complement Factor H on the Assembly and Decay of the Alternative Pathway C3 Proconvertase and C3 Convertase. J Biol Chem 291:8214-30 (2016). Read more (PubMed: 26903516) »

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