• Product nameAnti-C5 antibody [12F3]
    See all C5 primary antibodies
  • Description
    Mouse monoclonal [12F3] to C5
  • Specificityab17457 reacts with C5 in normal human plasma and no reaction is seen with plasma from C5 deficient patients.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native C5 protein isolated from human plasma.

  • EpitopeEpitope specificity differs from that of ab17930 but slightly overlaps, as determined by inhibition ELISA.



Our Abpromise guarantee covers the use of ab17457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/6000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 190 kDa. ab17457 recognises C5 in non reduced form only by western blot.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActivation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
      Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
    • Involvement in diseaseDefects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
      Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
    • Sequence similaritiesContains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Anaphylatoxin C5a analog antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
      • C5 antibody
      • C5a anaphylatoxin antibody
      • C5a antibody
      • C5b antibody
      • CO5_HUMAN antibody
      • Complement C5 alpha'' chain antibody
      • Complement C5 antibody
      • Complement component C5 antibody
      • CPAMD4 antibody
      • prepro-C5 antibody
      see all

    References for Anti-C5 antibody [12F3] (ab17457)

    ab17457 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. For ab11898, the antibody should recognize the whole mouse C5 chain; since this is a polyclonal antibody it is very likely that it recognizes both C5a and C5b. As the sequence of the immunogen used is commercially sensitive,...

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