Overview

  • Product nameAnti-C5 antibody
    See all C5 primary antibodies
  • Description
    Rabbit polyclonal to C5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human C5 aa 650-750 conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab92654)

  • Positive control
    • This antibody gave a positive signal in Human Plasma (Total Protein).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 188 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActivation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
      Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
    • Involvement in diseaseDefects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
      Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
    • Sequence similaritiesContains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Anaphylatoxin C5a analog antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
      • C5 antibody
      • C5a anaphylatoxin antibody
      • C5a antibody
      • C5b antibody
      • CO5_HUMAN antibody
      • Complement C5 alpha'' chain antibody
      • Complement C5 antibody
      • Complement component C5 antibody
      • CPAMD4 antibody
      • prepro-C5 antibody
      see all

    Anti-C5 antibody images



    • Predicted band size : 188 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: C5 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: A549 whole cell lysate (20 µg)
      Lane 4: HepG2 whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab66854 observed at 170 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab66854 was shown to recognize C5 when C5 knockout samples were used, along with additional cross-reactive bands. Wild-type and C5 knockout samples were subjected to SDS-PAGE. Ab66854 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10006 dilution for 1 hour at room temperature before imaging.

    • Anti-C5 antibody (ab66854) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 188 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 238 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-C5 antibody (ab66854)

    ab66854 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"