Overview

  • Product nameAnti-C5 / C5a antibody [557]
    See all C5 / C5a primary antibodies
  • Description
    Mouse monoclonal [557] to C5 / C5a
  • SpecificityThis antibody reacts with an epitope on C5 and C5a. The antibody 557 is capable to inhibit the binding of C5a to the C5a receptor through a competitive mechanism, it does not block the cleavage of C5 into C5a and C5b.
  • Tested applicationsSuitable for: WB, Inhibition Assay, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    BALB/c mice were immunized with human C5

  • EpitopeAb11876 recognizes an epitope located within the alpha-chain of C5 that corresponds to C5a.
  • Positive control
    • Recombinant C5

Properties

Applications

Our Abpromise guarantee covers the use of ab11876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
Inhibition Assay Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.

Target

  • FunctionActivation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • Involvement in diseaseComplement component 5 deficiency
    An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • Sequence similaritiesContains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anaphylatoxin C5a analog antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
    • C5 antibody
    • C5a antibody
    • CO5_HUMAN antibody
    • Complement C5 alpha'' chain antibody
    • Complement C5 antibody
    • Complement component C5 antibody
    • CPAMD4 antibody
    see all

Anti-C5 / C5a antibody [557] images

  • ELISA with ab11876 as capture antibody at 1/100, ab11878 as detection antibody at 1/250, diluted in carbonate buffer or PBS. Dilutions of commercial C5a were used as the standard.

    See Abreview

References for Anti-C5 / C5a antibody [557] (ab11876)

This product has been referenced in:
  • Wangdi T  et al. The Vi capsular polysaccharide enables Salmonella enterica serovar typhi to evade microbe-guided neutrophil chemotaxis. PLoS Pathog 10:e1004306 (2014). Mouse, Human . Read more (PubMed: 25101794) »
  • Kola A  et al. Epitope mapping of a C5a neutralizing mAb using a combined approach of phage display, synthetic peptides and site-directed mutagenesis. Immunotechnology 2:115-26 (1996). Read more (PubMed: 9373320) »

See all 2 Publications for this product

Product Wall

Thank you very much for your patience.

I have been checking with my lab colleagues the percentage of homology of the epitopes recognised by our anti C5 antibodies and the correspondent protein in bovine species. Unfortunately, I am afraid non...

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I have looked into this with the supplying lab:

Unfortunately we have no reference towards the statement. This has been communicated by a collaborator of the supplying lab upon the collaborator's intern...

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Thank you for your enquiry. Unfortunately we could not find this article in our file. Is it possible to send us this article? Nevertheless we can confirm the clone mentioned in this article is the same as our product. The ab11876 recognizes an e...

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Application ELISA
Sample Human Serum (Serum or EDTA plasma)
Specification Serum or EDTA plasma
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

Submitted Nov 02 2007

Thank you for your enquiry. Ab11876 recognizes an epitope located within the alpha-chain of C5 that corresponds to C5a. Thus it recognizes both C5 and C5a. The antibody inhibits binding of C5a to the C5a receptor through a competitive mechanism. It...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"