Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab46171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 188 kDa).

Target

  • RelevanceC5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • C5 antibody
    • Complement C5 antibody
    • Complement component 5 antibody
    • CPAMD4 antibody

Anti-C5 / C5b antibody images

  • Anti-C5 / C5b antibody (ab46171) at 1 µg/ml + Human Complement C5 Protein at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 188 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)

References for Anti-C5 / C5b antibody (ab46171)

ab46171 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"