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Full length protein corresponding to Human C5b-9.
Our Abpromise guarantee covers the use of ab66768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 23803904
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20688727|
|ELISA||Use at an assay dependent concentration.|
ab66768 staining C5b-9 in monkey retina cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton-X and blocked with 4% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Counterstained with DAPI. Magnification: 20X.
Immunohistochemical analysis of frozen tonsil tissue section labelling C5b-9 with ab66768 at a dilution of 1/10. Vascular endothelia and dendritic cells of germinal center have strong and intensive label.Anti-Complement component C5b-9 (human)