• Product name
    Anti-C5b-9 antibody [aE11]
    See all C5b-9 primary antibodies
  • Description
    Mouse monoclonal [aE11] to C5b-9
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC/IF, ELISA, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Horse, Human, Pig, Monkey, Baboon
  • Immunogen

    Full length protein corresponding to Human C5b-9.

  • Epitope
    ab66768 binds to the neoepitope exposed on C9 when incorporated into TCC (terminal complement complex).



Our Abpromise guarantee covers the use of ab66768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 23803904

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 20688727
ELISA Use at an assay dependent concentration.
IHC-Fr 1/10.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells.
    • Cellular localization
    • Database links
    • Alternative names
      • C5 antibody
      • c5b 9 antibody
      • C6 antibody
      • C7 antibody
      • C8 antibody
      • C9 antibody
      • Complement component 5 antibody
      • Complement component 6 antibody
      • Complement component 7 antibody
      • Complement component 8 antibody
      • Complement component 9 antibody
      see all


    • ab66768 staining C5b-9 (red) in porcine liver tissue by Immunohistochemistry (Frozen sections).

      Tissue was taken from allotransplants of wild-type or a1,3-galactosyltransferase gene-knockout (GTKO) pig liver, 2 hours after grafting or at necroscopy (nec). Tissue was fixed with paraformaldehyde and blocked with 20% donkey serum for 1 hour at room temperature, before being incubated with primary antibody (1/100 in diluent).
    • ab66768 staining C5b-9 in monkey retina cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton-X and blocked with 4% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Counterstained with DAPI. Magnification: 20X.

      See Abreview

    • Immunohistochemical analysis of frozen tonsil tissue section labelling C5b-9 with ab66768 at a dilution of 1/10. Vascular endothelia and dendritic cells of germinal center have strong and intensive label.Anti-Complement component C5b-9 (human)




    This product has been referenced in:
    • Mohan RR  et al. Peptide redesign for inhibition of the complement system: Targeting age-related macular degeneration. Mol Vis 22:1280-1290 (2016). Read more (PubMed: 27829783) »
    • Nakamura A  et al. Initial pulmonary respiration causes massive diaphragm damage and hyper-CKemia in Duchenne muscular dystrophy dog. Sci Rep 3:2183 (2013). Read more (PubMed: 23851606) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
    Monkey Tissue sections (Retina)
    Yes - 0.5 Triton-X

    Abcam user community

    Verified customer

    Submitted Jun 10 2014

    Thank you for contacting us.

    The datasheet pdfs can be downloaded from Abcam website.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    I am very pleased to hear you would like to accept our offer and test ab6678 in rabbit, ab8878 in IHC-P, and ab11862 in rabbit. These codes will give you 1 free primary antibody per code before the expiration date. To redeem this offer, please submit a...

    Read More


    Sign up