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Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Anti-C5orf22 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-C5orf22 antibody (ab122828)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclear membrane. Recommended concentration of ab122828 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-C5orf22 antibody (ab122828)
All lanes : Anti-C5orf22 antibody (ab122828) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5orf22 antibody (ab122828)
ab122828 at 1/200 dilution staining C5orf22 in Paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
References for Anti-C5orf22 antibody (ab122828)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"