Overview

  • Product nameAnti-C5orf22 antibody
    See all C5orf22 primary antibodies
  • Description
    Rabbit polyclonal to C5orf22
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: IWFHPTWAQQ IREGRHHFLV GKDTSTTTIR VTSTDHYFLS DGLYVPEDQL ENQKPLQLDV IMVKPYKLCN NQEENDAVSS, corresponding to internal sequence amino acids 97-176 of Human C5orf22.

  • Positive control
    • Human urinary bladder tissue; RT 4, U 251 MG, Human liver and Human tonsil lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-C5orf22 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclear membrane. Recommended concentration of ab122828 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-C5orf22 antibody (ab122828) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab122828 at 1/200 dilution staining C5orf22 in Paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.

References for Anti-C5orf22 antibody (ab122828)

ab122828 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"