Overview

  • Product nameAnti-C5ORF4 antibody
  • Description
    Rabbit polyclonal to C5ORF4
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • HepG2 cell lysate, human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab60131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500.

Target

  • RelevanceC5ORF4 belongs to the Sterol Desaturase family. It's function remains unknown. The Sterol Desaturase family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis of a plant cuticular wax. These enzymes contain many conserved histidine residues. Members of this family are integral membrane proteins. C5ORF4 has been identified as a putative tumour suppressor.
  • Cellular localizationCell Membrane (Potential).
  • Database links
  • Alternative names
    • Chromosome 5 open reading frame 4 antibody
    • FLJ13758 antibody
    • Hypothetical protein LOC10826 antibody

Anti-C5ORF4 antibody images

  • Anti-C5ORF4 antibody (ab60131) at 0.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.

    Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling C5ORF4 with ab60131 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-C5ORF4 antibody (ab60131)

ab60131 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab60131.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"