Overview

  • Product name
    Anti-C5ORF44 antibody
  • Description
    Rabbit polyclonal to C5ORF44
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Pig, Rhesus monkey, Gorilla, Chinese hamster, Common marmoset, Orangutan
  • Immunogen

    Recombinant fragment: LGETFSSYIS VHNDSNQVVK DILVKADLQT SSQRLNLSAS NAAVAELKPD CCIDDVIHHE VKEIGTHILV CAVSY, corresponding to amino acids 76-150 of Human C5ORF44 (A5PLN9).

  • Positive control
    • WB: RT4 lysate (weak band), Human liver lysate IHC-P: Human prostate tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-C5ORF44 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab122413 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-C5ORF44 antibody (ab122413) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate
    10-20% SDS PAGE
  • ab122413 at 1/50 dilution staining C5ORF44 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

References for Anti-C5ORF44 antibody (ab122413)

ab122413 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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