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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Anti-C5ORF44 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-C5ORF44 antibody (ab122413)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab122413 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-C5ORF44 antibody (ab122413)
All lanes : Anti-C5ORF44 antibody (ab122413) at 1/250 dilution Lane 1 : RT4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human Plasma lysate Lane 4 : Human Liver lysate Lane 5 : Human Tonsil lysate 10-20% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5ORF44 antibody (ab122413)
ab122413 at 1/50 dilution staining C5ORF44 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
References for Anti-C5ORF44 antibody (ab122413)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"