Anti-C5orf45 antibody (ab150917)
Key features and details
- Rabbit polyclonal to C5orf45
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C5orf45 antibody -
Description
Rabbit polyclonal to C5orf45 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 99-186 of Human C5orf45 (UniProt ID: Q6NTE8).
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Positive control
- IHC-P: Human Fallopian tube and endometrium tissue; ICC: A431 whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
C5orf45 belongs to the UPF0544 family. -
Database links
- Entrez Gene: 51149 Human
- SwissProt: Q6NTE8 Human
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Alternative names
- Chromosome 5 open reading frame 45 antibody
- DKFZp686L2452 antibody
- LOC51149 antibody
see all
Images
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Immunohistochemical analysis of human Fallopian tube tissue labeling C5orf45 in the nucleus of glandular cells with ab150917 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of A431 (Human epidermoid carcinoma cell line) whole cells labeling C5orf45 in the plasma membrane and cytoplasm with ab150917 at 2 µg/ml.
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Immunohistochemical analysis of human endometrium tissue labeling C5orf45 in the nucleus of glandular cells with ab150917 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab150917 has been referenced in 3 publications.
- Wang YL et al. MRNIP condensates promote DNA double-strand break sensing and end resection. Nat Commun 13:2638 (2022). PubMed: 35551189
- Bennett LG et al. MRNIP is a replication fork protection factor. Sci Adv 6:eaba5974 (2020). PubMed: 32832601
- Staples CJ et al. MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response. Cell Rep 16:2565-75 (2016). PubMed: 27568553