The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1 - 1/10. Use 10µl of this concentration to label 106 cells in 100µl
ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionReceptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Post-translational modificationsSulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.