The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionReceptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Post-translational modificationsSulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
ab115296, at 10µg/ml, staining C5AR1 in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115296, at 10µg/ml, staining C5AR1 in Formalin-fixed, Paraffin-embedded Human lung tissue by Immunohistochemistry followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-C5R1 antibody (ab115296)
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