Overview

  • Product nameAnti-C5R1 antibody [S5/1]
    See all C5R1 primary antibodies
  • Description
    Mouse monoclonal [S5/1] to C5R1
  • SpecificityThe antibody has been shown to inhibit the binding of C5a to its receptor.
  • Tested applicationsSuitable for: Inhibition Assay, WB, Flow Cyt, Functional Studies, IHC-Pmore details
    Unsuitable for: IHC-Fr
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    This antibody was raised against a synthetic peptide derived from the N-terminus of C5a (amino acids 1-31).

  • Positive control
    • Whole blood granulocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab11867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Functional Studies Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for IHC-Fr.
  • Target

    • FunctionReceptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
    • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
    • Post-translational
      modifications
      Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANAPHYLATOXIN RECEPTOR antibody
      • C5a anaphylatoxin chemotactic receptor antibody
      • C5a anaphylatoxin receptor antibody
      • C5A antibody
      • C5a ligand antibody
      • C5a-R antibody
      • C5aR antibody
      • C5AR_HUMAN antibody
      • c5ar1 antibody
      • C5R1 antibody
      • CD88 antibody
      • CD88 antigen antibody
      • Complement component 5 receptor 1 antibody
      • Complement component 5 receptor 2 antibody
      • Complement component 5a receptor 1 antibody
      see all

    Anti-C5R1 antibody [S5/1] images

    • Human peripheral blood granulocytes stained with ab11867 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab11867, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood granulocytes.

    • Immunohistochemical analysis of Human renal tissue, staining C5R1 with ab11867.

      For antigen retrieval, slides were immersed in citric acid buffer (pH 6) and were treated in a microwave oven for 10 minutes. Samples were blocked in 3% normal goat serum fr 30 minutes before incubating with primary antibody (1/50) overnight at 4°C. Staining was detected using DAB.
    • Immunohistochemical analysis of Human spleen (left) and resting neutrophils (right), staining C5R1 with ab11867.

      For antigen retrieval, slides were immersed in citric acid buffer (pH 6) and were treated in a microwave oven for 10 minutes. Samples were blocked in 3% normal goat serum fr 30 minutes before incubating with primary antibody (1/5) overnight at 4°C. A FITC-conjugated goat anti-mouse IgG (1/60) was used as the secondary antibody.

    References for Anti-C5R1 antibody [S5/1] (ab11867)

    This product has been referenced in:
    • Yuan J  et al. C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. Arthritis Res Ther 14:R140 (2012). IHC-P ; Human . Read more (PubMed: 22691190) »
    • Conway Morris A  et al. C5a mediates peripheral blood neutrophil dysfunction in critically ill patients. Am J Respir Crit Care Med 180:19-28 (2009). Read more (PubMed: 19324972) »

    See all 3 Publications for this product

    Product Wall

    Thank you for your enquiry. Please note as far as we know this application is not described in an article yet. We received the information that this antibody is useful for Western blot from the researcher who provided us this antibody. They give us...

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    Thank you for your enquiry. For flow cytometry, the dilutions to be used depend on the detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. ...

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    Thank you for your question concerning the use of ab11867 in inhibition assays. The use of this antibody for inhibition studies is described in the following article. Opperman, M et al; Antibodies from myeloid panel that react with the C5a recept...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"