Anti-C6orf130 antibody (ab122032)

Overview

  • Product nameAnti-C6orf130 antibody
  • Description
    Rabbit polyclonal to C6orf130
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to N terminal amino acids 1-78 (MASSLNEDPE ASRITYVKGD LFACPKTDSL AHCISEDCRM GAGIAVLFKK KFGGVQELLN QQKKSGEVAV LKRDGRYI) of Human C6orf130.

  • Positive control
    • Human small intestine tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-C6orf130 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and nucleoli. Recommended concentration of ab122032 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122032 at 1/200 dilution staining C6orf130 in paraffin-embedded Human small intestine by Immunohistochemistry.

References for Anti-C6orf130 antibody (ab122032)

ab122032 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (lymphoblastoid)
Loading amount 30 µg
Specification lymphoblastoid
Gel Running Conditions Reduced Denaturing (12.5)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Nov 12 2012

DISCOUNT CODE: 0000

Expiration date: 13. November 2012
I am very pleased to hear your customer would like to accept our offer and test ab122032 in WB.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"