Overview

  • Product nameAnti-C6orf211 antibody
    See all C6orf211 primary antibodies
  • Description
    Rabbit polyclonal to C6orf211
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 52-172 of Human C6orf211.

  • Positive control
    • Human testis tissue; HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20ºC.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-C6orf211 antibody images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121158 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121158 at 1/50 dilution staining C6orf211 in Paraffin-emebedded Human Testis tissue by Immunohistochemistry.

    Note: strong cytoplasmic positivity in cells in seminiferus ducts.
  • All lanes : Anti-C6orf211 antibody (ab121158) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : HEK293T Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

References for Anti-C6orf211 antibody (ab121158)

ab121158 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"