Overview

  • Product nameAnti-C6orf222 antibody
  • Description
    Rabbit polyclonal to C6orf222
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SFRRAFYHKK HTSKEPRRAG AAGAASPEAR RPKRPSFLPL CVGGHRPSTS SSLDPEDLEC REPLPAEGEP VVISEAPSQ, corresponding to internal sequence amino acids 438-516 of Human C6orf222.

  • Positive control
    • Human testis tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab122471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-C6orf222 antibody images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122471 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122471, at 1/200 dilution, staining C6orf222 in paraffin-embedded Human testis tissue by Immunohistochemitry.

References for Anti-C6orf222 antibody (ab122471)

ab122471 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (human bronchial epithelial cell, lung)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 60 µg
Specification human bronchial epithelial cell, lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Miss. Hsin-Ju Wang

Verified customer

Submitted Dec 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"