Anti-C6orf57 antibody (ab122196)
Key features and details
- Rabbit polyclonal to C6orf57
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-C6orf57 antibody -
Description
Rabbit polyclonal to C6orf57 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to C terminal amino acids 23-107 (SPLLCHSLRK TSSSQGGKSE LVKQSLKKPK LPEGRFDAPE DSHLEKEPLE KFPDDVNPVT KEKGGPRGPE PTRYGDWERK GRCID) of Human C6orf57.
-
Positive control
- IHC-P: Human prostate, testis, small intestine, and fallopian tube; ICC: Human cell line A-431.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
|
IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ICC
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Sequence similarities
Belongs to the UPF0369 family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 135154 Human
- SwissProt: Q5VUM1 Human
- Unigene: 418495 Human
-
Alternative names
- C6orf57 antibody
- CF057_HUMAN antibody
- Chromosome 6 open reading frame 57 antibody
- UPF0369 protein C6orf57 antibody
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labelling C6orf57 with ab122196 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunocytochemistry/immunofluorescence analysis of human cell line A-431 labelling C6orf57 with ab122196 at 2 µg/mL.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling C6orf57 with ab122196 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling C6orf57 with ab122196 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling C6orf57 with ab122196 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab122196 has not yet been referenced specifically in any publications.