Overview

  • Product nameAnti-C6orf57 antibody
  • Description
    Rabbit polyclonal to C6orf57
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to C terminal amino acids 23-107 (SPLLCHSLRK TSSSQGGKSE LVKQSLKKPK LPEGRFDAPE DSHLEKEPLE KFPDDVNPVT KEKGGPRGPE PTRYGDWERK GRCID) of Human C6orf57.

  • Positive control
    • Human duodenum tissue; C6orf57 over-expression HEK293T lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-C6orf57 antibody images

  • ab122196 at 1/25 dilution staining C6orf57 in Paraffin-embedded Human Duodenum tissue by Immunohistochemistry.
  • All lanes : Anti-C6orf57 antibody (ab122196) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : C6orf57-transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

    Developed using the ECL technique

References for Anti-C6orf57 antibody (ab122196)

ab122196 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (MEF, C2C12)
Loading amount 25 µg
Specification MEF, C2C12
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 27 2012

Application Western blot
Sample Human Cell lysate - whole cell (HepG2, 293T)
Loading amount 15 µg
Specification HepG2, 293T
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"