Overview

  • Product name
    Anti-C6orf72 antibody
  • Description
    Rabbit polyclonal to C6orf72
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Protein Epitope Signature Tag, corresponding to amino acids 37-145 of Human C6orf72.

  • Positive control
    • Human gall bladder

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59.9% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli and nucleus but not nucleoli. Recommended concentration of ab121528 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121528, at 1/50 - 1/200 dilution, staining C6orf72 in paraffin-embedded Human gall bladder by Immunohistochemistry.
  • All lanes : Anti-C6orf72 antibody (ab121528)

    Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
    Lane 2 : RT-4 lysate
    Lane 3 : U-251 MG lysate
    Lane 4 : Human Plasma
    Lane 5 : Liver lysate
    Lane 6 : Tonsil lysate

References

ab121528 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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