Anti-C6orf97 antibody (ab97814)

Overview

  • Product nameAnti-C6orf97 antibody
    See all C6orf97 primary antibodies
  • Description
    Rabbit polyclonal to C6orf97
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 442-701 of Human C6orf97 (NP_079335).

  • Positive control
    • A549 whole cell lysate; HeLa, HepG2 and HCT116 cell lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab97814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/500 - 1/3000. Predicted molecular weight: 82 kDa.

Target

Anti-C6orf97 antibody images

  • Anti-C6orf97 antibody (ab97814) at 1/1000 dilution + A549 whole cells lysate at 30 µg

    Predicted band size : 82 kDa
  • ab14404 staining C6orf97 - in Human Breast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 10% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in PBS + 10% FBS) for 1 hour at 21°C. A diluted(1/2500) Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-C6orf97 antibody (ab97814)

ab97814 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (breast)
Specification breast
Fixative Paraformaldehyde
Permeabilization Yes - 0.1 % triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

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Verified customer

Submitted Sep 28 2012

Of course we will prolong the discount code for your customer under this circumstances for the time it takes until the antibody is delivered. They just need to quote this number XXXXX next to the discount code in the "AdditionalNotes" section.
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I am very pleased to hear your customer would like to test ab97814 Anti-C6orf97 antibody in ICC/ IF. This code will give them 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forICC/ IF and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"